Plasmid

Part:BBa_I713005:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEB-SC101


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2102
    Illegal SpeI site found at 258
    Illegal PstI site found at 2062
    Illegal PstI site found at 3252
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal PstI site found at 2062
    Illegal PstI site found at 3252
    Illegal NotI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3039
    Illegal BamHI site found at 252
    Illegal XhoI site found at 2090
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2102
    Illegal SpeI site found at 258
    Illegal PstI site found at 2062
    Illegal PstI site found at 3252
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2102
    Illegal SpeI site found at 258
    Illegal PstI site found at 2062
    Illegal PstI site found at 3252
    Illegal NgoMIV site found at 1708
    Illegal NgoMIV site found at 2428
    Illegal NgoMIV site found at 3703
    Illegal AgeI site found at 1868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4602
    Illegal SapI.rc site found at 3552
    Illegal SapI.rc site found at 3762


Design Notes

the SC101 ori was clone with PCR.


Source

pBHR91 pEASY-B cloning vector

References